Cytological study of Сhloromonas typhlos (Chlamydomonadaceae, Chlorophyta) from North-Western Russia

O. N. Boldina

DOI: https://doi.org/10.31111/nsnr/2017.51.5

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Abstract

The strain of biflagellated green monad, selected from the specimen gathered in the Novgorod Region was studied by light and electron microscopy. The species was identified on the base of coincidence of the majority of specific light and ultrastructural signs revealed in both the strain SAG 26.86 of Chloromonas typhlos (Gerloff) Matsuzaki et al. and in studied strain. On LM level the cells are ellipsoid, some asymmetric, 12–17 μm long, 8–13 μm wide, with an apical, hardly distinguished hemispherical papilla flattened on the top. Chloroplast parietal, cup-shaped, thickened on one side, with big lobes, irregular incisions and fissures on the surface. These and other features are characteristic of the type and the studied strain of C. typhlos, excluding stigma observed in the studied strain only. On the ultrastructural level, large thylakoid packs, similar with chloroplast thylakoids, were observed in pyrenoids of both strains of C. typhlos. The detected pyrenoid type was never discovered in chlamydomonads earlier. It is the first record of C. typhlos for the Novgorod Region and the whole Russia, and adds the list of «bloom-forming» species. The strain was deposited in the collection of microalgae strains in the Institute of Biology of Komi Scientific Centre under the name SYKOA Ch-063-17.

Keywords: green monads, Chloromonas, Chlamydomonas, light microscopy, transmission electron microscopy, Novgorod Region, Russia

Section: Algae

How to cite

Boldina O. N. 2017. Cytological study of Сhloromonas typhlos (Chlamydomonadaceae, Chlorophyta) from North-Western Russia. Novosti Sist. Nizsh. Rast. 51: 5–11. https://doi.org/10.31111/nsnr/2017.51.5

References

Boldina O. N. 2011. Classification principles of pyrenoids in green monads. Vodorosli: taksonomiya, ekologiya i ispolzovanie v monitoringe [The Algae: taxonomy, ecology and use in the monitoring]. Yekaterinburg: 34–41. (In Russ. with Engl. abstract).

Boldina O. N. 2016. Сhlamydomonads (Chlamydomonadaceae) from St. Petersburg and the Leningrad Region: current knowledge and new records. Novosti Sist. Nizsh. Rast. 50: 5–22. (In Russ. with Engl. abstract).

Ettl H. 1983. Chlorophyta I (Phytomonadina). Süsswasser flora von Mitteleuropa. Bd 9. Stuttgart: 807 S.

Gerloff J. 1940. Beiträge zur Kenntnis der Variabilität und Systematik der Gattung Chlamydomonas. Arch. Protistenk. 94(3): 311–502.

Matsuzaki R., Hara Y., Nozaki Н. 2012. A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia. 51(1): 74–85. https://doi.org/10.2216/11-18.1

Morel-Laurens N. M. L., Bird D. J. 1984. Effects of cell division on the stigma of wild-type and an «eyeless» mutant of Chlamydomonas. J. Ultrastruct. Res. 87(1): 46–61. https://doi.org/10.1016/S0022-5320(84)90115-1

Novakovskaya I. V., Patova E. N. Collection of living microalgae strains of the Institute of Biology, Komi Science Centre, Ural Branch, RAS, and its perspective using. 2012. Izv. Komi nauchn. tsentra UrO RAN. 2(10): 36–41. (In Russ.).

Pröschold T., Marin B., Schlösser U. G., Melkonian M. 2001. Molecular phylogeny and taxonomic revision of Chlamydomonas (Chlorophyta). I. Emendation of Chlamydomonas Ehrenberg and Chloromonas Gobi and description of Oogamochlamys gen. nov. and Lobochlamys gen. nov. Protist. 152(4): 265–300. https://doi.org/10.1078/1434-4610-00068

Rundina L. A. 2006. Division Chlorophyta. Vodorosli, vyzyvayushchie «tsvetenie» vodoemov Severo-Zapada Rossii [Bloom-forming algae in water bodies of Northwestern Russia]. Moscow: 219–291. (In Russ.).

Schlösser U. G. 1994. SAG — Sammlung von Algenkulturen at the University of Göttingen. Catalogue of strains. Bot. Acta. 107(1/2): 113–186. https://doi.org/10.1111/j.1438-8677.1994.tb00784.x