Revision of collections of Hebeloma spp. from the high-altitude Altay Mountains (Altay Republic) and report of a new to Russia species of H. excedens


N. V. Filippova, I. A. Gorbunova


DOI: https://doi.org/10.31111/nsnr/2025.59.1.F33


Abstract

Eight Hebeloma specimens from the high-altitude zone of Altai Mountains (West Siberia) were analyzed using morphological and molecular methods. Three species were identified (H. alpinum, H. dunense, H. excedens), including the previously unreported in Russia species H. excedens. The detailed morphological description and ecological data on H. excedens based on the studied collections are provided.


Keywords: Agaricales, Hymenogastraceae, taxonomy, West Siberia


Section: Fungi


How to cite

Filippova N. V., Gorbunova I. A. 2025. Revision of collections of Hebeloma spp. from the high-altitude Altay Mountains (Altay Republic) and report of a new to Russia species of H. excedens. Novosti sistematiki nizshikh rastenii 59(1): F33–F40. https://doi.org/10.31111/nsnr/2025.59.1.F33


Received: 5 February 2025. Accepted: 31 March 2025. Published: 6 April 2025


References

Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990. Basic Local Alignment Search Tool. Journal of Molecular Biology 215(3): 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2

Bartlett P., Eberhardt U., Beker H. J. 2022. Demystifying Hebeloma: introducing hebeloma.org and its database. IMA Fungus 13:18. https://doi.org/10.1186/s43008-022-00105-2

Beker H. J., Eberhardt U., Vesterholt J. 2010. Hebeloma hiemale Bres. in arctic/alpine habitats. North American fungi 5(5): 51–65. https://doi.org/10.2509/naf2010.005.0054

Beker H. J., Eberhardt U., Schütz N., Gulden G. 2018. A review of the genus Hebeloma in Svalbard. Mycoscience 59(4): 303–309. https://doi.org/10.1016/j.myc.2017.12.001

Bolshakov S., Kalinina L., Palomozhnykh E., Potapov K., Ageyev D., Arslanov S., Filippova N., Palamarchuk M., Tomchin D., Voronina E. 2021. Agaricoid and boletoid fungi of Russia: the modern country-scale checklist of scientific names based on literature data. Biological communication 66(4): 316–325. https://doi.org/10.21638/spbu03.2021.404

Clémençon H. 2009. Methods for working with macrofungi laboratory cultivation and preparation of larger fungi for light microscopy. Eching: 88 p.

Cripps C., Eberhardt U., Schütz N., Beker H. J., Evenson V. S., Horak E. 2019. The genus Hebeloma in the Rocky Mountain alpine zone. MycoKeys 46: 1–54. https://doi.org/10.3897/mycokeys.46.32823

Dizkirici A., Kalmer A., Acar I. 2022. New Turkish records of Hebeloma excedens and H. geminatum and confirmation of H. celatum. Mycotaxon 137: 123–134. https://doi.org/10.5248/137.123

Eberhardt U., Ronikier A., Schütz N., Beker H. J. 2015. The genus Hebeloma in the alpine belt of the Carpathians including two new species. Mycologia 107: 1285–1303. https://doi.org/10.3897/mycokeys.79.63363

Eberhardt U., Beker H. J., Borgen T., Knudsen H., Schütz N., Elborne S. A. 2021. A survey of Hebeloma (Hymenogastraceae) in Greenland. MycoKeys 79: 17–118. https://doi.org/10.3897/mycokeys.79.63363

Eberhardt U., Schütz N., Bartlett P., Beker H. J. 2022. 96 North American taxa sorted – Peck’s Hebeloma revisited. Mycologia 114:2: 337–387. https://doi.org/10.1080/00275514.2021.2012063

Gardes M., Bruns T. D. 1993. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

Gorbunova I., Filippova N. 2025. Fungarium of Central Siberian Botanical Garden, NSK (curated by Gorbunova Irina A.). Version 1.37. Yugra State University Biological Collection (YSU BC). Occurrence dataset https://doi.org/10.15468/upme2c accessed via GBIF.org (Data of access: 22 I 2025).

Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307–321. https://doi.org/10.1093/sysbio/syq010

Hoang D. T., Chernomor O., Haeseler A. V., Minh B. Q., Vinh L. S. 2017. UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35(2): 518–522. https://doi.org/10.1093/molbev/msx281

Kalyaanamoorthy S., Minh B. Q., Wong T. K. F., Haeseler A., Jermiin L. S. 2017. ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285

Kazutaka K., John R., Kazunori D. Y. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4): 1160–1166. https://doi.org/10.1093/bib/bbx108

Knudsen H., Vesterholt J. 2018. Funga Nordica. Agaricoid, boletoid, clavarioid, cyphelloid and gastroid genera. Copenhagen: 1083 p.

Koichiro T., Glen S., Sudhir K. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022–3027. https://doi.org/10.1093/molbev/msab120

Kotkova V. M., Afonina O. M., Alverdiyeva S. M., Anissimova O. V., Bragin A. V., Cherenkova N. N., Davydov E. A., Dongak D. A.-S., Doroshina G. Ya., Efremov A. N., et al. 2024. New cryptogamic records. 13. Novosti sistematiki nizshikh rastenii 58(1): R1–R45. https://doi.org/10.31111/nsnr/2024.58.1.R1

Matheny P. B., Curtis J. M., Hofstetter V., Aime M. C., Moncalvo J.-M., Ge Z.-W., Yang Z. L., Slot J. C., Ammirati J. F., Baroni T. J., et al. 2006. Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98: 982–995. https://doi.org/10.1080/15572536.2006.11832627

Miller O. K. Jr. 1998. Hebeloma in the Arctic and alpine tundrain Alaska. Arctic andalpine mycology 5. Proceedings of the 5th International Symposium on Arcto-Alpine Mycology (Labytnangi, Russia, 15–27 Aug 1996). Ekaterinburg: 86–97.

Nguyen L. T., Schmidt H. A., Haeseler A. V., Minh B. Q. 2015. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution 32: 268–274. https://doi.org/10.1093/molbev/msu300

Peck C. H. 1872. Report of the Botanist (1870). Annual Report on the New York State Museum of Natural History 68: 41–108.

Rambaut А. 2006–2018. FigTree v1.4.4. https://github.com/rambaut/figtree/releases/tag/v1.4.4 (Date of access: 1 I 2025).

Vesterholt J. 2005. The genus Hebeloma. Fungi of northern Europe. Vol. 3. Tilsit: 146 p.

White T. J., Bruns T., Lee S., Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. London: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1



Supplementary materials

Supplementary file. GenBank accession numbers of DNA sequences used in this study
NSNR_2025_59(1)_Philippova_Supplement.pdf