Kirill Demchenko

Kirill Demchenko

Author Identifiers: Web of Science ResearcherID, Scopus Author ID, Russian Science Citation Index, ORCID iD, Google Scholar

Academic degree, academic title: Candidate of Biological Sciences (PhD)

Position: Head of the Laboratory


Research interests:

  • Plant growth and development. Development of plant root systems. Cellular and molecular mechanisms of plant meristem function. Lateral root initiation and formation.
  • Plant cell proliferation, cell cycle and endoreduplication in plants. Hormonal regulation and patterning in plants. Cytoskeleton and signaling in plants.
  • Plant–microbe symbiosis. Mycorrhiza. Nitrogen fixing root nodules (rhizobial and actinorhizal).

Education and work experience:
1988 – 1994, student, Department of Botany, Biology and soil sciences faculty, St.-Petersburg State university, Russia.
1994, Honours M.S. Degree in Biology, Department of Higher plants, St.-Petersburg State university, Russia.
1994 – 1997, Ph.D. Student, Laboratory of Plant Anatomy and morphology, Komarov Botanical Institute, Russian Academy of sciences.
1999, Candidate of Sciences (Biology) Degree (analogue of Ph.D.), Komarov Botanical Institute, Russian Academy of Sciences, Thesis title: Cell proliferation during lateral root initiation. (supervisors: Prof. Valery G. Grif and Dr. Nikolay P. Demchenko).

1994 – 1997, Ph.D. student in the Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences.
1994 – 1999, Assistant, Department of Pharmacognosy, St.-Petersburg State Chemical Pharmaceutical Academy.
1997 – 2004, Scientific Researcher in the Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences.
2001 – 2003, Postdoctoral researcher in the Department of Plant Biochemistry, Albrecht von Haller Institute for Plant Sciences, University of Göttingen, Germany (supervisor – Prof. Katharina Pawlowski).
2004 – 2014, Senior Scientist in the Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences, Russia.
2006 – present, Leading Research Scientist in the Laboratory of Molecular and Cellular Biology, Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology (ARRIAM).
2014 – 2018, Leading Research Scientist in the Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences, Russia.
2018 – present, Head of the Laboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, Russia.

Grants and research projects:
Russian Science Foundation
2020–2022 – Grant № 20-16-00115 «Small signal peptides in systemic regulation of fast root branching strategy of cucumber and buckwheat root systems», project leader.
2016–2018 – Grant № 16-16-00089 «The key regulatory factors in the molecular machinery of productivity and resistance enhancement in cucurbits and rice», project leader.

Russian Foundation for Basic Research
2019–2021 – Grant № 19-04-01079-а «Key transcription factors of the LBD family and their targets in the regulation of root system branching of Cucurbitaceae», project leader.
2014–2016 – Grant № 14-04-01413-а «Local morphogenetic triggers and the specification of lateral root founder cell identity in the parental root meristem of Cucurbitaceae», project leader.
2011–2013 – Grant № 11-04-02022-а «Structural and molecular genetic aspects of lateral root initiation in the main root meristem», project leader.
2008–2010 – Grant № 08-04-01710-а «Structural and genetic bases of competence of root nodule cells to stably internally accommodate a bacterial endosymbiont», project leader.

Programs supported by the Presidium of the Russian Academy of Sciences
2012 – 2014 – «Study of root system branching using genetic transformation of economically important Cucurbitaceae crops by Agrobacterium rhizogenes strains», project leader.
2015 – 2020 – «The role of molecular genetic mechanisms of meristem functioning in the plasticity of root systems of higher plants», project leader.

Professional memberships:

Selected publications:

  1. Kiryushkin A.S., Ilina E.L., Guseva E.D., Pawlowski K., Demchenko K.N. Lateral root initiation in cucumber (Cucumis sativus): what does the expression pattern of Rapid Alkalinization Factor 34 (RALF34) tell us?. International Journal of Molecular Sciences. 2023. 24(9): 8440. │
  2. Shumilina J., Kiryushkin A.S., Frolova N., Mashkina V., Ilina E.L., Puchkova V.A., Danko K., Silinskaya S., Serebryakov E.B., Soboleva A., Bilova T., Orlova A., Guseva E.D., Repkin E., Pawlowski K., Frolov A., Demchenko K.N. Integrative proteomics and metabolomics analysis reveals the role of small signaling peptide Rapid Alkalinization Factor 34 (RALF34) in cucumber roots. International Journal of Molecular Sciences. 2023. 24(8): 7654. │
  3. Kiryushkin A.S., Ilina E.L., Guseva E.D., Pawlowski K., Demchenko K.N. Hairy CRISPR: Genome editing in plants using hairy root transformation. Plants. 2022. 11(1): 51. │
  4. Gogolev Y.V., Ahmar S., Akpinar B.A., Budak H., Kiryushkin A.S., Gorshkov V.Y., Hensel G., Demchenko K.N., Kovalchuk I., Mora-Poblete F., Muslu T., Tsers I.D., Yadav N.S., Korzun V. OMICs, epigenetics, and genome editing techniques for food and nutritional security. Plants. 2021. 10(7): 1423. │
  5. Voitsekhovskaja O.V., Melnikova A.N., Demchenko K.N., Ivanova A.N., Dmitrieva V.A., Maksimova A.I., Lohaus G., Tomos A.D., Tyutereva E.V., Koroleva O.A. Leaf epidermis: The ambiguous symplastic domain. Frontiers in Plant Science. 2021. 12(1474): 695415. │
  6. Dolgikh E.A., Kusakin P.G., Kitaeva A.B., Tsyganova A.V., Kirienko A.N., Leppyanen I.V., Dolgikh A.V., Ilina E.L., Demchenko K.N., Tikhonovich I.A., Tsyganov V.E. Mutational analysis indicates that abnormalities in rhizobial infection and subsequent plant cell and bacteroid differentiation in pea (Pisum sativum) nodules coincide with abnormal cytokinin responses and localization. Annals of Botany. 2020. 125(6): 905–923. │
  7. Ilina E.L., Kiryushkin A.S., Demchenko K.N. Features of fluorescent protein application to study the root system development of cucurbits (Cucurbitaceae). Agricultural Biology (Sel'skokhozyaistvennaya Biologiya). 2020. 55(5): 1040-1055. │
  8. Tsyganov V.E., Kitaeva A.B., Demchenko K.N. 2019. Comparative analysis of tubulin cytoskeleton rearrangements in nodules of Medicago truncatula and Pisum sativum. In The model legume Medicago truncatula, edited by F. J. de Bruijn. Hoboken, NJ: John Wiley & Sons, Inc. 547-543. │
  9. Demina I.V., Maity P.J., Nagchowdhury A., Ng J.L.P., van der Graaff E., Demchenko K.N., Roitsch T.G., Mathesius U., Pawlowski K. Accumulation of and response to auxins in roots and nodules of the actinorhizal plant Datisca glomerata compared to the model legume Medicago truncatula. Frontiers in Plant Science. 2019. 10:1085. │
  10. Nguyen T.V., Wibberg D., Vigil-Stenman T., Berckx F., Battenberg K., Demchenko K.N., Blom J., Fernandez M.P., Yamanaka T., Berry A.M., Kalinowski J., Brachmann A., Pawlowski K. Frankia-enriched metagenomes from the earliest diverging symbiotic Frankia cluster: they come in teams. Genome Biology and Evolution. 2019. 11(8): 2273–2291. │
  11. Kiryushkin A.S., Ilina E.L., Puchkova V.A., Guseva E.D., Pawlowski K., Demchenko K.N. Lateral root initiation in the parental root meristem of cucurbits: Old players in a new position. Frontiers in Plant Science. 2019. 10:365. │
  12. Shishova M., Puzanskiy R., Gavrilova O., Kurbanniazov S., Demchenko K., Yemelyanov V., Pendinen G., Shavarda A., Gavrilenko T. Metabolic alterations in male-sterile potato as compared to male-fertile. Metabolites. 2019. 9(2): 24(1-18). │
  13. Kitaeva A.B., Kusakin P.G., Demchenko K.N., Tsyganov V.E. Key methodological features of tubulin cytoskeleton studies in nodules of legume plants. Agricultural Biology. 2018. 53(3): 634–644. │
  14. Ilina E.L., Kiryushkin A.S., Semenova V.A., Demchenko N.P., Pawlowski K., Demchenko K.N. Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player?. Annals of Botany. 2018. Special Issue on Developmental Plant Cell Biology, 122(5), 873–888.│
  15. Zdyb A., Salgado M.G., Demchenko K.N., Brenner W.G., Płaszczyca M., Stumpe M., Herrfurth C., Feussner I., Pawlowski K. Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules. PLOS ONE. 2018. 13(1): e0190884. │
  16. Ilina E.L., Kiryushkin A.S., Tsyganov V.E., Pawlowski К., Demchenko K.N. Molecular, genetic and hormonal outlook in root branching. Agricultural Вiology. 2017. 52(5): 856–868. │
  17. Kitaeva A.B., Demchenko K.N., Tikhonovich I.A., Timmers A.C.J., Tsyganov V.E. Comparative analysis of the tubulin cytoskeleton organization in nodules of Medicago truncatula and Pisum sativum: Bacterial release and bacteroid positioning correlate with characteristic microtubule rearrangements. New Phytologist. 2016. 210(1): 168–183. │
  18. Lebedeva M.A., Tvorogova V.E., Vinogradova A.P., Gancheva M.S., Azarakhsh M., Ilina E.L., Demchenko K.N., Dodueva I.E., Lutova L.A. Initiation of spontaneous tumors in radish (Raphanus sativus): Cellular, molecular and physiological events. Journal of Plant Physiology. 2015. 173: 97–104. │
  19. Demchenko K.N., Voitsekhovskaja O.V., Pawlowski K. Plasmodesmata without callose and calreticulin in higher plants - open channels for fast symplastic transport? Frontiers in Plant Science. 2014. 5(74): 1–5. │
  20. Schubert M., Koteyeva N.K., Zdyb A., Santos P., Voitsekhovskaja O.V., Demchenko K.N., Pawlowski K. Lignification of cell walls of infected cells in Casuarina glauca nodules that depend on symplastic sugar supply is accompanied by reduction of plasmodesmata number and narrowing of plasmodesmata. Physiologia Plantarum. 2013. 147(4): 524–540. │
  21. Demchenko N.P., Kalimova I.B., Demchenko K.N. Restoration of cell growth and proliferation in the wheat roots after their inhibition by nickel sulfate. Russian Journal of Plant Physiology. 2013. 60(5): 640–651.
  22. Pawlowski K., Demchenko K.N. The diversity of actinorhizal symbiosis. Protoplasma. 2012. 249(4): 967–979. │
  23. Osipova M.A., Mortier V., Demchenko K.N., Tsyganov V.E., Tikhonovich I.A., Lutova L.A., Dolgikh E.A., Goormachtig S. WUSCHEL-RELATED HOMEOBOX5 gene expression and interaction of CLE peptides with components of the systemic control add two pieces to the puzzle of autoregulation of nodulation. Plant Physiology. 2012. 158(3): 1329–1341. │
  24. Ilina E.L., Logachov A.A., Laplaze L., Demchenko N.P., Pawlowski K., Demchenko K.N. Composite Cucurbita pepo plants with transgenic roots as a tool to study root development. Annals of Botany. 2012. 110(2): 479–489. │
  25. Demchenko K., Zdyb A., Feussner I., Pawlowski K. Analysis of the subcellular localisation of lipoxygenase in legume and actinorhizal nodules. Plant Biology. 2012. 14(1): 56–63. │
  26. Zdyb A., Demchenko K., Heumann J., Mrosk C., Grzeganek P., Gцbel C., Feussner I., Pawlowski K., Hause B. Jasmonate biosynthesis in legume and actinorhizal nodules. New Phytologist. 2011. 189(2): 568–579. │
  27. Schubert M., Koteyeva N., Wabnitz P., Santos P., Bьttner M., Sauer N., Demchenko K., Pawlowski K. Plasmodesmata distribution and sugar partitioning in nitrogen-fixing root nodules of Datisca glomerata. Planta. 2011. 233(1): 139–152. │
  28. Rashidi B., Mehrabi S., Demchenko K., Pawlowski K. The Casuarina glauca metallothionein I promoter in nodulated transgenic hairy roots of the actinorhizal plant Datisca glomerata. Functional Plant Biology. 2011. 38(9): 728–737.
  29. Berry A.M., Mendoza-Herrera A., Guo Y.-Y., Hayashi J., Persson T., Barabote R., Demchenko K., Zhang S., Pawlowski K. New perspectives on nodule nitrogen assimilation in actinorhizal symbioses. Functional Plant Biology. 2011. 38(9): 645–652.
  30. Demchenko N.P., Kalimova I.B., Demchenko K.N. Effect of nickel at high concentration on proliferation of quiescent center cells and initiation of lateral root primordia in wheat seedlings. Russian Journal of Plant Physiology. 2010. 57(3): 438–447.
  31. Voroshilova V.A., Demchenko K.N., Brewin N.J., Borisov A.Y., Tikhonovich I.A. Initiation of a legume nodule with an indeterminate meristem involves proliferating host cells that harbour infection threads. New Phytologist. 2009. 181(4): 913–923. │
  32. Voitsekhovskaja O.V., Rudashevskaya E.L., Demchenko K.N., Pakhomova M.V., Batashev D.R., Gamalei Y.V., Lohaus G., Pawlowski K. Evidence for functional heterogeneity of sieve element-companion cell complexes in minor vein phloem of Alonsoa meridionalis. Journal of Experimental Botany. 2009. 60(6): 1873–1883. │
  33. Stumpe M., Göbel C., Demchenko K., Hoffmann M., Klösgen R.B., Pawlowski K., Feussner I. Identification of an allene oxide synthase (CYP74C) that leads to formation of ±-ketols from 9-hydroperoxides of linoleic and linolenic acid in below-ground organs of potato. The Plant Journal. 2006. 47(6): 883–896. │
  34. Ktitorova I.N., Demchenko N.P., Kalimova I.B., Demchenko K.N., Skobeleva O.V. Cellular analysis of UV-B-induced barley root subapical swelling. Russian Journal of Plant Physiology. 2006. 53(6): 824–837.
  35. Karatygin I., Snigirevskaya N., Demchenko K. Species of the genus Glomites as plant mycobionts in Early Devonian ecosystems. Paleontological Journal. 2006. 40(5): 572–579.
  36. Demchenko N.P., Kalimova I.B., Demchenko K.N. Effect of nickel on growth, proliferation, and differentiation of root cells in Triticum aestivum seedlings. Russian Journal of Plant Physiology. 2005. 52(2): 220–228.
  37. Jeong J., Suh S., Guan C., Tsay Y., Moran N., Oh C.J., An C.S., Demchenko K.N., Pawlowski K., Lee Y. A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family. Plant Physiology. 2004. 134(3): 969–978. │
  38. Demchenko K.N., Winzer T., Stougaard J., Parniske M., Pawlowski K. Distinct roles of Lotus japonicus SYMRK and SYM15 in root colonization and arbuscule formation. New Phytologist. 2004. 163(2): 381–392.
  39. Doll J., Hause B., Demchenko K.N., Pawlowski K., Krajinski F. A member of the germin-like protein family is a highly conserved mycorrhiza-specific induced gene. Plant and Cell Physiology. 2003. 44(11): 1208–1214. │
  40. Demchenko K.N., Demchenko N.P. Changes of root structure in connection with the development of lateral root primordia in wheat and pumpkins. Recent Advances of Plant Root Structure and Function: Proceedings of the 5th International Symposium on Structure and Function of Roots. Stará Lensná, Slovakia, 1998. O. Gašparíková, Čiamporová M., Mistrík I., Baluška F. Dordrecht, Springer Netherlands. 2001. 39–47.
  41. Demchenko N.P., Demchenko K.N. Resumption of DNA synthesis and cell division in wheat roots as related to lateral root initiation. Russian Journal of Plant Physiology. 2001. 48(6): 755–763.
  42. Demchenko K.N., Demchenko N.P. Early stages of lateral root development in Triticum aestivum L. Acta Phytogeographica Suecica: Plant root systems and natural vegetation. Persson H., Baitulin I.O. Uppsala, Opulus Press AB. 1996. 81: 71–75.